| 1 | Yulee High SchoolYULEE | Other | 1,431 |
| 2 | Yulee Middle SchoolYULEE | Middle | 1,215 |
| 3 | Wildlight ElementaryYULEE | Primary | 1,164 |
| 4 | Fernandina Beach High SchoolFERNANDINA BEACH | High | 980 |
| 5 | West Nassau County High SchoolCALLAHAN | High | 933 |
| 6 | Hilliard Middle-Senior HighHILLIARD | High | 783 |
| 7 | Callahan Middle SchoolCALLAHAN | Middle | 708 |
| 8 | Hilliard Elementary SchoolHILLIARD | Primary | 696 |
| 9 | Fernandina Beach Middle SchoolFERNANDINA BEACH | Middle | 689 |
| 10 | Callahan Elementary SchoolCALLAHAN | Primary | 657 |
| 11 | Emma Love Hardee ElementaryFERNANDINA BEACH | Primary | 645 |
| 12 | Yulee Primary SchoolYULEE | Primary | 638 |
| 13 | Southside Elementary SchoolFERNANDINA BEACH | Primary | 619 |
| 14 | Yulee Elementary SchoolYULEE | Primary | 599 |
| 15 | Callahan Intermediate SchoolCALLAHAN | Primary | 591 |
| 16 | Bryceville Elementary SchoolBRYCEVILLE | Primary | 222 |
| 17 | Nassau County Community SchoolFERNANDINA BEACH | High | 90 |
| 18 | Nassau Virtual FranchiseFERNANDINA BEACH | Other | 86 |
| 19 | Nassau Virtual Instruction ProgramFERNANDINA BEACH | Other | 0 |
| 20 | Nassau Adult and Career CenterFERNANDINA BEACH | High | 0 |
| 21 | Nassau Virtual Instruction Program (District Provided)FERNANDINA BEACH | Other | 0 |
| 22 | Contracted Residential ServicesFERNANDINA BEACH | -- | 0 |